Table 3.
Quantitative nisin A-binding assay results with the nsrX deletion mutant and nsrX-expressing cells
| Strain | Genotype | Fraction | Nisin A bindinga (μg/ml) | P valueb |
|---|---|---|---|---|
| MM3019 | nsrX mutant | Bound | 10.7 ± 0.11 | |
| MM3055 | nsrX+ in nsrX mutant | Bound | 17.8 ± 0.23 | 0.008 |
| MM3019 | nsrX mutant | Unbound | 21.1 ± 0.70 | |
| MM3055 | nsrX+ in nsrX mutant | Unbound | 16.5 ± 0.94 | 0.015 |
a
Mean ± standard deviation binding activity, as measured by liquid chromatography/mass spectrometry.
b
Statistical significance for the difference between the nsrX deletion mutant versus the nsrX-expressing strain, based on Student's t test.