Table 1.
Plasmids and strains used in this studya
| Plasmid or strain | Relevant characteristic(s) | Source |
|---|---|---|
| Plasmids | ||
| pUC18 | E. coli cloning vector; Apr | TaKaRa |
| pZGX01 | Wild plasmid in P. acidipropionici ATCC 4875 | This study |
| pZGX04 | E. coli-PAB shuttle vector Apr in E. coli and Cmr in PAB; derived from pUC18, pZGX02, and cml/cmx PCR product | This study |
| pZGX04-gldA | gldA expression vector | This study |
| Strains | ||
| E. coli JM109 | Clone host | TaKaRa |
| E. coli JM110 | dam and dcm mutant conferring no methylation of DNA | Stratagene |
| K. pneumonia subsp. pneumoniae ATCC 12657 | Carries gldA | ATCC |
| P. acidipropionici ATCC 4875 | Carries plasmid pZGX01 | ATCC |
| P. acidipropionici ATCC 4965 | No native plasmid; electrotransformation | ATCC |
| P. acidipropionici ATCC 25562 | No native plasmid; electrotransformation | ATCC |
| P. jensenii ATCC 4868 | No native plasmid; electrotransformation; transformable by shuttle vector pZGX04 | ATCC |
| P. jensenii ATCC 4868 (pZGX04-gldA) | P. jensenii ATCC 4868 carries pZGX04-gldA | This study |
| P. jensenii ATCC 4870 | No native plasmid; electrotransformation; transformable by shuttle vector pZGX04 | ATCC |
| P. freudenreichii subsp. freudenreichii ATCC 6207 | No native plasmid; electrotransformation; transformable by shuttle vector pZGX04 | ATCC |
| P. freudenreichii subsp. shermanii ATCC 9614 | No native plasmid; electrotransformation | ATCC |
| P. thoenii ATCC 4872 | No native plasmid; electrotransformation | ATCC |
| P. thoenii ATCC 4874 | No native plasmid; electrotransformation | ATCC |
a
Apr, ampicillin selection; Cmr, chloramphenicol selection; cml/cmx, complete cml(A) and cmx(A) genes; ATCC, American Type Culture Collection, Manassas, VA.