Fig 2.

Induction of the citrate catabolic pathway of Lb. casei ATCC 334. (A) Citrate consumption rate of resting cells grown in batches in mMRS medium supplemented with 10 mM calcium chloride and/or 30 mM sodium citrate as indicated. Cells were harvested at late exponential growth (OD₆₆₀ = 0.6). (B) Growth curves of Lb. casei ATCC 334 in microtiter plates in mMRS medium without further additions (open circles) or in the presence of 10 mM Ca²⁺ (filled squares), 30 mM citrate (filled triangles), both calcium and citrate (open squares), or both calcium and 10 mM glucose (filled circles). Means of three independent experiments are shown.