Fig 4.

Carbohydrate/Ca²⁺-citrate cometabolism. (A) Citrate consumption rates of resting cells of Lb. casei ATCC 334 grown in mMRSCitCa without further additions and with the addition of glucose and galactose as indicated. The cells were harvested at the indicated OD₆₆₀. The average values and standard deviations of three independent experiments are shown. (B, C) Growth curves of Lb. casei ATCC 334 during glucose/Ca²⁺-citrate (B) and galactose/Ca²⁺-citrate (C) cometabolism. The curves represent growth in mMRS medium with 10 mM calcium chloride (filled circles) supplemented with 28 mM carbohydrate (open circles) and with 28 mM carbohydrate and 30 mM citrate (filled triangles).