Fig 4.

Efficient silencing of MsALP and APN1 by feeding specific dsRNA to neonate M. sexta larvae. (A) Transcript abundance was determined using qRT-PCR and Sybr green. Bars represent the means and standard errors for 5 individual midguts. (B) APN Western blot of midgut proteins (5 μg) prepared from individual control larvae (lane 1) and 5 apn dsRNA-fed larvae (lanes 2 to 8). (C) ALP Western blot of midgut proteins (5 μg) prepared from individual control larvae (lane 1) and 5 alp dsRNA-fed larvae (lanes 2 to 6).