Strains |
|
|
DH5α |
F− (ϕ80lacZΔM15) Δ(lacZYA-argF)U169 recA1 endA1
hsdR17 (rK− mK+) phoA
supE44
thi-1
gyrA96
relA1
|
Laboratory collection |
Rosetta (DE3) |
lon and ompT protease-deficient E. coli carrying pRARE; expresses six rare tRNAs which facilitate expression of genes that contain rare E. coli codons; Camr; p15a ori |
Merck KGaA, Darmstadt, Germany |
SPC105 |
MC4100 (ΔlacU169 araD rpsL
relA
thi
flbB) containing a chromosomal PmarII::lacZ fusion at the λ attachment site; wild-type mar locus |
6 |
SPC107 |
SPC105 with a 39-kb deletion that includes the mar locus |
23 |
AG100 |
argE3 thi-1 rpsL xyl mtl supE44 λ lysogen |
24 |
AG112 |
AG100 marR mutant |
25 |
Plasmids |
|
|
pGEM-T Easy |
Vector for direct cloning of PCR fragments; Ampr
|
Promega, Madison, WI |
pET28a |
Expression cloning vector; Kanr; the transcription of the cloned gene is driven by the T7 RNA polymerase and controlled by the LacI repressor; ori ColE1 |
Merck KGaA |
pET21b |
Expression cloning vector; Ampr; the transcription of the cloned gene is driven by the T7 RNA polymerase and controlled by the LacI repressor; ori ColE1 |
Merck KGaA |
pACT7Sp |
T7 RNA polymerase regulated by lacUV5; Spr; this plasmid is used in conjunction with pET28a; ori p15a |
26 |
pET13a-G95S |
pET13a carrying G95S variant of marR; Kanr
|
22 |
pET13a-D26N |
pET13a carrying D26N variant of marR; Kanr
|
22 |
pVDMarR |
pET28a carrying wild-type marR; Kanr
|
This study |
pVD57 |
pET28a carrying P57A variant of marR; Kanr
|
This study |
pVD72 |
pET28a carrying T72A variant of marR; Kanr
|
This study |
pVD86 |
pET28a carrying R86A variant of marR; Kanr
|
This study |
pVD74 |
pET28a carrying M74A variant of marR; Kanr
|
This study |
pVD77 |
pET28a carrying R77A variant of marR; Kanr
|
This study |
pVD5772 |
pET28a carrying P57A-T72A variant of marR; Kanr
|
This study |
pVD7477 |
pET28a carrying M74A-R77A variant of marR; Kanr
|
This study |
pVD26 |
pET28a carrying D26N variant of marR; Kanr
|
This study |
pVD95 |
pET28a carrying G95S variant of marR; Kanr
|
This study |
pVD16 |
pET28a carrying R16A variant of marR; Kanr
|
This study |
pET28a-MarR-H19A |
pET28a carrying H19A variant of marR; Kanr
|
This study |
pET28a-MarR-K44A |
pET28a carrying K44A variant of marR; Kanr
|
This study |
pVDOR |
pET21b carrying ompR nucleotide sequence cloned in frame with a polyhistidine tag at the C-terminal end |
This study |