Table 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Relevant genotype or description | Source or reference |
|---|---|---|
| Strains | ||
| Escherichia coli | ||
| DH5α | F− ϕ80dlacZΔM15 Δ(lacZYA-argF)U169 recA1 endA1 hsdR17(rK− mK+) phoA supE44 thi-1 gyrA96 relA1 tonA | Invitrogen Corp. |
| BL21 | F− ompT hsdSB (rB− mB−) gal dcm rne131 (DE3) | Invitrogen Corp. |
| P. aeruginosa | ||
| PAO1 | Wild type | B. H. Holloway |
| ΔbrlR mutant | PAO1, ΔbrlR (PA4878) | 28 |
| PAO255 | PAO1, ΔmexAB-oprM-ΔmexEF-oprN | 67 |
| Plasmids | ||
| pCR2.1-TOPO | TA cloning vector; Kmr Apr | Invitrogen Corp. |
| pRK2013 | Helper plasmid for triparental mating; mob tra; Kmr | 75 |
| pJN105 | Arabinose-inducible gene expression vector; pBRR-1 MCS; araC-PBAD; Gmr | 41 |
| pMJT1 | Arabinose-inducible gene expression vector; pUCP18 MCS; araC-PBAD; Ampr/Carbr | 76 |
| pJN-brlR | brlR cloned into pJN105 | 28 |
| pMJT-brlR-V5/His6 | brlR-V5/His6 cloned into pMJT1 | 28 |
| pmexAB-oprM | mexAB-oprM operon, cloned into pJN105 at NheI-SacI; Gmr | This study |
| pmexEF-oprN | mexEF-oprN operon, cloned into pMJT1 at NheI-SacI; Ampr/Carbr | This study |