Table 2.
Primers used in this study
| Oligonucleotide | Sequencea |
|---|---|
| RT-PCR, PCR, or cloning | |
| mreB-for | CTGTCGATCGACCTGGG |
| mreB-rev | CAGCCATCGGCTCTTCG |
| 16S rDNA_f | GACTCCTACGGGAGGCAGCAGT |
| 16S rDNA_r | GTATTACCGCGGCTGCTGGCAC |
| mexA-for | CAGCAGCTCTACCAGATCGAC |
| mexA-rev | GTATTGGCTACCGTCCTCCAG |
| mexE-for | GTCATCGAACAACCGCTG |
| mexE-rev | GTCGAAGTAGGCGTAGACC |
| mexAB-oprM-for NheI | GCGCGCGCTAGCGTAAGTATTTTGCCTGCCTTCTTC |
| mexAB-oprM-rev SacI | GCGCGCGAGCTCGATCAAGCCTGGGGATCTTCC |
| mexEF-oprN-for | GCGCGCGCTAGCGAGTCAAGCATGGAACAGTCATC |
| mexEF-oprN-rev | GCGCGCGAGCTCCTGGAGTGGCCGATTTCCATC |
| EMSA and streptavidin bead binding assays | |
| PmexAFb | GTAGTTCATTGGTTTGGCC |
| PmexAR | CATAGCGTTGTCCTCATG |
| PmexEFb | GGATCAGCATGTTCATCG |
| PmexER | CTGTTCCATGCTTGACTC |
| mexA_F4 | TTTTGCCTGCCTTCTTCG |
| mexA_R4 | TCGCTTTTTCCGCACCCG |
| pscE_GS_F | AAGGCGGTCTCGGCATTCTTTC |
| pscF_GS_R | CCACGGTATCGAGGGTATTC |
| ChIP or ChIP enrichment | |
| PmexAF | GTAGTTCATTGGTTTGGCC |
| PmexAR | CATAGCGTTGTCCTCATG |
| PmexEF | GGATCAGCATGTTCATCG |
| PmexER | CTGTTCCATGCTTGACTC |
| pscE_GS_F | AAGGCGGTCTCGGCATTCTTTC |
| pscF_GS_R | CCACGGTATCGAGGGTATTC |
a
Restriction sites are underlined.
b
Primer was biotinylated. Unbiotinylated primers were used for competition.