Fig 1.
The FeoC protein is necessary for high cellular levels of the FeoB Fe(II) transporter under low-oxygen and -iron conditions. Salmonella strains were grown aerobically (high O2) or anaerobically (low O2) in LB medium (high Fe) or in LB medium supplemented with 0.2 mM deferoxamine (low Fe). By conducting immunoblot analysis, levels of the FeoB protein were determined in wild-type (WT, 14028s), fur deletion (Δfur, JH352), fnr deletion (Δfnr, HK708), and feoC deletion (ΔfeoC, JH363) strains (A) and in the wild-type strain (14028s) and the feoC deletion strain (ΔfeoC, JH363) carrying the empty plasmid vector pUHE21-2lacIq (pUHE) (14) or FeoC-expression plasmid (plac-FeoC) (B). The band indicated with an asterisk corresponds to a protein displaying cross-reactivity against anti-FeoB antibody and serving as an internal loading control.