Fig 5.

FeoC-dependent FeoB production enables Salmonella to take up Fe(II) under anaerobic conditions with low iron. ⁵⁵Fe(II) uptake levels were determined in Salmonella strains that were grown aerobically (high O₂) or anaerobically (low O₂) in LB (high Fe) or LB containing 0.2 mM deferoxamine (low Fe). Bacterial strains used in the experiments were wild-type (WT, 14028s), feoB deletion (ΔfeoB, JH362), and feoC deletion (ΔfeoC, JH363) strains (A) and wild-type (WT, 14028s) and feoB deletion (ΔfeoB, JH362) strains and feoC deletion (ΔfeoC, JH363) and feoBC deletion (ΔfeoBC, HK715) strains carrying the empty plasmid vector pUHE21-2lacI^q (pUHE) or FeoC-expression plasmid (plac-FeoC) (B). Values are the means and SD of three independent experiments.