Table 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Description | Source or reference |
|---|---|---|
| E. coli DH5α | Strain used for general cloning procedures | Invitrogen |
| H. ducreyi strains | ||
| 35000HP | Human-passaged variant of strain 35000; parental strain | 56 |
| 35000HPΔcpxR | 35000HPcpxR in-frame deletion mutant containing a chloramphenicol resistance cartridge | 19 |
| 35000HPΔcpxA | 35000HPcpxA unmarked, in-frame deletion mutant | 20 |
| Plasmids | ||
| pRB157 | pLS88 derivative containing an ΩAmp cartridge, followed by a BglII site for insertion of promoter sequences and a promoterless GFP cassette derived from pGreenTIR | 20 |
| pKF1 | pRB157 derivative containing the lspB promoter region | 20 |
| pKF2 | pRB157 derivative containing the dsrA promoter region | 20 |
| pDG2 | pRB157 derivative containing the HD0182 promoter region | This study |
| pDG3 | pRB157 derivative containing the fimA promoter region | This study |
| pDG4 | pRB157 derivative containing the HD0427 promoter region | This study |
| pDG5 | pRB157 derivative containing the HD1123 promoter region | This study |
| pDG6 | pRB157 derivative containing the HD1278 promoter region | This study |
| pDG7 | pRB157 derivative containing the fis promoter region | This study |