Fig 3.

Gene dosage-dependent regulation of inflammatory genes in lung by β-arrestin-2 following polymicrobial infection. RNA was obtained from lung (A) and liver (B) tissue samples from wild-type (WT), β-arrestin-2 homozygous knockout (β-arr2^−/−), and β-arrestin-2 heterozygous (β-arr2^+/−) mice at 6 h after polymicrobial infection. mRNA expression of the indicated inflammatory genes was performed using real-time qRT-PCR as described in Materials and Methods. The values were converted to fold naive WT for each experiment. *, P < 0.05; **, P < 0.01; using a t test or Mann-Whitney test. n = 3 for naive mice and 7 to 9 for septic mice of each genotype.