Fig 1.

The LVS migR::TrgTn, LVS ΔtrmE, and LVS ΔcphA mutants have decreased iglA reporter expression. Mutations in migR and fevR were constructed by TargeTron (TrgTn) insertion (Sigma). Mutations in trmE and cphA were generated by homologous recombination. An iglA-lacZ reporter plasmid was cryotransformed into each of the F. tularensis LVS mutants, and β-galactosidase assays were performed to compare gene expression in each of the mutant strains to that in the wild type (Wt). The difference between iglA-lacZ expression in each mutant strain and the LVS parent was statistically significant (P < 0.001).