Table 2.

Select CREB and Wnt/β-catenin pathway peptide phosphorylation sites differentially phosphorylated by M. avium subsp. paratuberculosis-infected intestinal lysates from CMI responder and antibody responder calves^a

Compartment	CMI responders				Antibody responders
	LEF1 (Q9UJU2); T155 or S166 (↑)		β-Catenin (P35222); Y142, T41/45, or S675 (↑)		ADCY8 (P40145) Y406 (↑)		p300 (Q09472); S893 or S1834 (↑)		PP2CA (P67775); T304/7 (↑)
	FC	P	FC	P	FC	P	FC	P	FC	P
1 Prox	1.5	0.08	1.7	0.13	—	—	−1.6	0.10	1.2	0.31
2 Prox	5.5	0.04	1.6	0.06	−1.4	0.36	−1.6	0.23	−1.4	0.1
2 Dist	3.9	0.07	1.7	0.18	−1.0	0.48	−1.5	0.04	−1.5	0.3
3 Prox	−2.0	0.03	−3.4	0.0004	16	0.02	6.2	0.005	1.5	0.04
3 Dist	−1.8	0.26	−3.2	0.02	6.4	0.04	3.4	0.04	2.3	0.12
4 Dist	−2.2	0.05	−1.8	0.006	6.5	0.01	2.0	0.1	1.2	0.14

^aThe fold change (FC) and probability value from a t test between the transformed treatment (infected lysate) intensities and the transformed control (uninfected lysate) intensities for the indicated peptide (Uniprot accession numbers are given in parentheses) and residue(s) are shown. Prox, proximal compartment; Dist, distal compartment. —, inconsistently phosphorylated.