Fig 2.
Interaction of H. pylori with porcine and rat mucins. Mucin microarrays were probed with fluorescently labeled H. pylori organisms. Three wild-type strains and strain G27ΔbabA were used. G27ΔbabA does not express the BabA outer membrane protein, which mediates binding to the Lewisb blood group antigen. H. pylori was cultured on agar as described in Materials and Methods, harvested, and resuspended in brain heart infusion broth supplemented with 10% FBS. Cultures were incubated at 37°C for 4 h, at which time bacteria were harvested, washed, and resuspended in PBS to an OD600 of 1.0. Strain G27ΔbabA was cultured in the presence of 10 μg/ml of kanamycin. The histogram shows the mean fluorescence intensities from duplicate subarrays on three replicate microarray slides; values for each subarray are medians for six feature replicates. Error bars indicate the standard deviations of the means for three microarray slides.