Fig 9.

Interactions of H. pylori and C. jejuni with the non-mucin-containing fractions of HT29-MTX-E12 cell mucus. (A) Mucus from HT29-MTX-E12 cells was separated on a CsCl gradient and was then divided into 1-ml fractions. A sample of each non-mucin-containing fraction was blotted onto a PVDF membrane, which was probed with an antibody against Lewis^b. Shown are 3 fractions taken from the top of the density gradient. (B) TLC separation and orcinol staining of glycolipids extracted from secreted HT29-MTX-E12 mucus. (C) TLC-separated glycolipids were probed with antibodies against Lewis^b (Le B) and sialyl-Lewis^x (Sialyl Le X). (D) TLC plates containing glycolipids were probed for the binding of either C. jejuni strain 81-176 or H. pylori strain 26695 or J99. When identical TLC plates that were not overlaid with microorganisms were probed with either anti-H. pylori or anti-C. jejuni and secondary antibodies, no signals were detected.