Table 2.
Structures of O-glycans released from E-12 mucins, described by HILIC-HPLC and exoglycosidase digestion arrays
Glucose unit (GU) values are averages of triplicates, with a standard deviation of 0.06. Asterisks indicate proposed isomeric structures.
Structures are represented using the Oxford system for monosaccharides and linkages (Fig. 6, key) (59). ND, not determined.
The enzymes used were bovine testis β-galactosidase (BTG), Arthrobacter ureafaciens sialidase (ABS), Streptococcus pneumoniae sialidase (NAN1), Streptococcus pneumoniae β-N-acetylglucosaminidase (GUH), Streptococcus pneumoniae β-galactosidase (SPG), bovine kidney α-fucosidase (BKF), and almond meal α-fucosidase (AMF).