Fig 3.

ΔfeoB mutant shows impaired growth in medium containing low levels of iron. Growth of the wild-type LVS, the ΔfeoB and ΔfslC mutants, and their corresponding complemented strains (c. ΔfeoB and c. ΔfslC) was assessed in MH II broth supplemented with ferric pyrophosphate (MH+) (A), MH II broth without addition of ferric pyrophosphate (MH−) (B), Che-CDM containing 7.2 μM FeSO₄ (C), or Che-CDM containing 360 nM FeSO₄ (D). Growth of the bacterial strains was quantified by measuring the OD₆₀₀ at various times. All experiments were repeated two more times with similar results.