Fig 8.
ΔfeoB mutant is defective for colonization of the liver, lungs, and spleen of infected mice. C3H/HeN mice were inoculated intradermally with a sublethal dose (∼3 × 105 CFU) of the wild-type LVS, the ΔfeoB or ΔfslC mutant, or their complemented strains (c. ΔfslC and c. ΔfeoB). Organs were harvested 3 days after infection, and the CFU/g of liver (A), lungs (B), and spleen (C) were determined. The data are combined from three independent experiments. A total of 8 mice per group in two experiments was used in studies of the ΔfeoB and c. ΔfeoB strains; samples from nine organs were lost to contamination. A total of 5 mice per group was used in one experiment with the ΔfslC and c. ΔfslC strains. The bars denote medians ± SD. *, P < 0.05; **, P < 0.01; ***, P < 0.001.