Fig 4.

Influence of Mn²⁺ and Zn²⁺ on RpoS and OspC production in B. burgdorferi strain B31. Strain B31-A3 was grown at 37°C in Chelex-treated BSK-II medium adjusted to pH 7.0, with addition of various amounts of Mn²⁺ and/or Zn²⁺. Samples were harvested at the late logarithmic phase, and cell lysates were detected by Coomassie blue-stained SDS-PAGE gel (A) or by immunoblotting using antisera against FlaB, RpoS, and BosR (B). Data are representative of two separate experiments performed in triplicate.