Fig 5.

Deletion of bmtA resulted in elevated OspC and BosR production. Wild-type B. burgdorferi strain 297 (A) or strain B31-A3 (B) and the isogenic bmtA mutant or the complemented strain (bmtA-comp) were grown in BSK-II medium (pH 7.5) at 37°C and harvested at the late logarithmic phase. Equal amounts of whole-cell lysates were separated by Coomassie blue-stained SDS-PAGE gel (left panels), or probed for FlaB or BosR protein by immunoblotting analyses (right panels). Data shown are representative of two separate experiments performed in duplicate.