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Schematic diagram depicting the construction of pBSV28-3G. The relevant restriction enzyme recognition sites used for cloning have been indicated, as well as the paralogous gene family designations for bbh26, bbh27, bbh28, and bbh29 in boxes above the genes. The family 62, 50, 32, and 49 genes are responsible for the replication and partitioning functions required for plasmid maintenance in B. burgdorferi (11).
