Table 1.
Detection of M. genitalium in primate specimens by quantitative PCR and culturea
| Specimen type |
M. genitalium detected in specimen by the indicated method at the following time: |
||||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Wk 0 |
Wk 1 |
Wk 2 |
Wk 3 |
Wk 4 |
Wk 5 |
Wk 8 |
|||||||||||||||
| qPCRb | H-broth culturec | Vero cocultured | qPCR | H-broth culture | Vero coculture | qPCR | H-broth culture | Vero coculture | qPCR | H-broth culture | Vero coculture | qPCR | H-broth culture | Vero coculture | qPCR | H-broth culture | Vero coculture | qPCR | H-broth culture | Vero coculture | |
| Vaginal swab | − | − | − | + | − | + | + | − | + | − | − | + | + | + | + | − | − | + | − | − | − |
| Cervical swab | − | − | − | + | − | + | + | + | + | + | − | + | + | − | + | − | − | − | − | − | + |
| Cervical cytobrush | ND | ND | ND | ND | ND | ND | + | ND | − | − | − | + | + | − | + | − | − | − | +f | − | + |
| Pocket 1e (PBS) | − | − | − | − | − | − | − | − | − | − | − | − | |||||||||
| Pocket 2 (PBS) | − | − | − | − | − | − | − | − | − | − | − | − | |||||||||
| Pocket 3 (Mg) | − | − | − | + | + | + | − | − | − | − | − | − | |||||||||
| Pocket 4 (Mg) | − | − | − | + | + | + | − | − | − | + | − | − | |||||||||
| Pocket 5 (Mg) | + | + | + | + | + | − | − | − | − | − | − | − | |||||||||
Specimens collected at week 11 (three weeks after azithromycin treatment) were negative by qPCR and H-broth culture (data not shown).
M. genitalium genomes in 2.4 μl of primate specimen as determined by quantitative PCR. Samples were considered positive (+) if the number of genomes was more than twice that of preinoculation specimens. −, not detected; ND, not determined.
Growth in 3 ml H broth was considered positive (+) if the color changed from red to orange or yellow, indicating the production of acid, in any of three tubes inoculated with 1:10, 1:100, and 1:1000 dilutions of specimen, and then incubated for 3 weeks. −, not detected; ND, not determined.
Growth in 8.5-ml Vero cocultures was determined by qPCR by detecting M. genitalium genomes in 20 μl of culture supernatant collected 7, 14, and 21 days after inoculation with 0.2 ml of specimen. Positive (+) cultures demonstrated >200-fold increase in M. genitalium genomes. −, not detected; ND, not determined.
A total of 20 abdominal salpingeal pockets were inoculated with PBS or M. genitalium (Mg) as indicated. Five salpingeal pockets were excised at weeks 1, 2, 3, and 4; no pockets were collected for weeks 0, 5, and 8.
The week 8 cervical cytobrush specimen was further analyzed for sequence variants in mgpB region B.