Fig 10.
Effect of CsrABb on the members of the flgK motility operon. Separated proteins of strains wt, mt, and ct (A) and strains ct, 7D, and 8S (B) propagated under conditions that mimic the tick midgut before (23°C/pH 7.6; lanes 1) and after (37°C/pH 6.8; lanes 2) a blood meal were electrotransferred and subjected to immunoblot analysis using antisera against FlaB, BB0180 (FlbF), BB0181 (FlgK), BB0182 (FlgL, indicated by arrow), BB0183 (FliW), and P66 (loading control). Note the absence of any detectable levels of FliW in the csrABb mutant and 7D strain. Levels of FliW were elevated in the 8S mutant compared to the ct strain under unfed-tick conditions. Levels of FlbF and FlgK were unchanged in the strains tested. Blots represent one of two independent experiments. Molecular masses in kilodaltons are indicated to the left.