Fig 3.
Immunoblot analysis of RpoS and rpoS-regulated proteins in the csrABb mutant grown with supplemental acetate. The wt, mt, and ct strains were propagated in BSK-II medium with 0 (lanes 1), 30 (lanes 2), or 90 (lanes 3) mM supplemental acetate at 37°C. Equivalent levels of proteins were loaded on SDS–12.5% PAGE and were either stained with Coomassie blue (A) or electrotransferred to PVDF membranes (B). The blots were probed with antisera against CsrABb, RpoS, OppA5, BBA64, and DbpA and developed using an ECL system. Blots represent one of three independent experiments. Molecular masses in kilodaltons are indicated to the left.