Fig 5.

Schematic representation of the mutations generated in CsrA_Bb. (A) The cis-complemented strain (ct) was generated to restore a functional native copy of csrA_Bb. The residues indicated in gray are the conserved amino acids that have been shown to interact with small RNA molecule CsrB in E. coli. (B) Schematic representation of the DNA construct lacking the sequence encoding the last seven amino acids of CsrA_Bb (7D). (C) Site-specific changes were made to change 8 critical residues (underlined) to alanines (8S). All three complemented strains were counterselected for resistance to gentamicin conferred by P_flgB-Gent^R.