Fig 9.

Immunoblot analysis of ct, 7D, and 8S mutant strains of B. burgdorferi grown with supplemental acetate. Total proteins from ct, 7D, and 8S strains were grown under unfed (23°C/pH 7.6) (A and B) or fed (37°C/pH 6.8) (C and D) tick conditions with 0 (lanes 1), 30 (lanes 2), and 90 (lanes 3) mm of supplemental acetate. Equivalent amounts of proteins were separated on an SDS–12.5% PAGE gel (A and B). Separated proteins were electrotransferred and subjected to immunoblot analysis (B and D) using antisera against Pta, AckA, OppA5, and P66 (loading control). Blots represent one of three independent experiments. Molecular masses in kilodaltons are indicated to the left.