Table 1.
Oligonucleotide primers used for MLST
| Oligonucleotide primer | Target genetic marker, oligonucleotide orientation | Sequence (5′→3′) | Reference |
|---|---|---|---|
| fD1mod | 16S rRNA gene, forward | AGAGTTTGATCYTGGYTYAG | This study |
| fD1 | 16S rRNA gene, forward | AGAGTTTGATCCTGGCTCAG | 1 |
| rP2 | 16S rRNA gene, reverse | ACGGCTACCTTGTTACGACTT | 1 |
| Bart/16-23F | 16S-23S rRNA intergenic spacer region (ISR), forward | TTGATAAGCGTGAGGTCGGAGG | 5 |
| Bart/16-23R | 16S-23S rRNA intergenic spacer region (ISR), reverse | CAAAGCAGGTGCTCTCCCAG | 5 |
| BhCS.781p | GltA gene, forward | GGGGACCAGCTCATGGTGG | 4 |
| BhCS.1137n | GltA gene, reverse | AATGCAAAAAGAACAGTAAACA | 4 |
| prAPT0244 | RpoB gene, forward | GATGTGCATCCTACGCATTATGG | This study |
| prAPT0245 | RpoB gene, reverse | AATGGTGCCTCAGCACGTATAAG | This study |