Table 2.
PCR programs used for MLST in this study
| Step | Temp (°C), duration of step with oligonucleotide primers: |
|
|---|---|---|
| fD1mod + rP2 | fD1 + rP2, Bart/16-23F + Bart/16-23R, BhCS.781p + BhCS.1137n, prAPT0244 + prAPT0245 | |
| 1, Initial denaturation | 94, 3 min | 95, 1 min |
| 2, Denaturation | 94, 30 s | 95, 30 s |
| 3, Annealing | 55, 30 s | 55, 40 s |
| 4, Extension | 72, 1 min (37)a | 72, 1 min (39)a |
| 5, Final extension | 72, 7 min | 72, 10 min |
| 6, Hold | 4, indefinitely | 4, indefinitely |
a
The value in parentheses is the number of cycles of steps 2 to 4.