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. 2013 Aug;51(8):2625–2632. doi: 10.1128/JCM.00992-13

Table 1.

Genes and primers used in this study

HK gene Annotation Locus tag Position in the C. pecorum E58 genomea Primer Sequence (5′ → 3′) Annealing temp (°C) Amplicon size (bp) Size of sequence analyzed (bp) Reference or source
gatA Glutamyl-tRNA amidotransferase subunit A G5S_0628 629005–629429 MJgatA1F GCTTTAGAGTTGAGAGAAGCT 54 512 425 30
MJgatA1R GATCCTCCTGTATCTGATCC 54 512 425 30
oppA_3 Oligonucleotide-binding protein G5S_0967 952191–952671 MJoppA1F ATGTGCAAGATCCCAGTGGG 58 605 483 30
MJoppA1R GGCGCTACTTGTTATGGG 58 605 483 This study
hflX GTP binding protein G5S_0597 579741–580175 MJhflX1F TGAGGAGATCTCTGCATCG 58 607 435 This study
MJhflX1R ATCTTCATGCAAAGCAGCC 58 607 435 This study
gidA Glucose-inhibited division protein A G5S_0430 429868–429395 MJgidA1F GCGTCACAACAAAAGAAGGC 60 560 474 This study
MJgidA1R TGACGCTGTATATCACACGG 60 560 474 This study
enoA Enolase G5S_0242 254779–255159 MJenoA1F CCTATGATGAACCTTATCAATGG 58 431 381 30
MJenoA1R TCTTCCTCCGCTAAGCCATCC 58 431 381 30
hemN Oxygen-independent coproporphyrinogen III oxidase G5S_0144 150138–150569 MJhemN1F GATCGCGATAGAGATAGACCC 54 634 432 This study
MJhemN1R ATCTTCTCCTGATAGATATCG 54 634 432 This study
fumC Fumarate hydratase class II G5S_0015 18169–17705 MJfumC1F TGATTAAGAAATGTGCAGC 54 572 465 This study
MJfumC1R CCTTCAGGTACATTAAGCC 54 572 465 30
a

GenBank accession number CP002608.