Table 1.

Genes and primers used in this study

HK gene	Annotation	Locus tag	Position in the C. pecorum E58 genomea	Primer	Sequence (5′ → 3′)	Annealing temp (°C)	Amplicon size (bp)	Size of sequence analyzed (bp)	Reference or source
gatA	Glutamyl-tRNA amidotransferase subunit A	G5S_0628	629005–629429	MJgatA1F	GCTTTAGAGTTGAGAGAAGCT	54	512	425	30
				MJgatA1R	GATCCTCCTGTATCTGATCC	54	512	425	30
oppA_3	Oligonucleotide-binding protein	G5S_0967	952191–952671	MJoppA1F	ATGTGCAAGATCCCAGTGGG	58	605	483	30
				MJoppA1R	GGCGCTACTTGTTATGGG	58	605	483	This study
hflX	GTP binding protein	G5S_0597	579741–580175	MJhflX1F	TGAGGAGATCTCTGCATCG	58	607	435	This study
				MJhflX1R	ATCTTCATGCAAAGCAGCC	58	607	435	This study
gidA	Glucose-inhibited division protein A	G5S_0430	429868–429395	MJgidA1F	GCGTCACAACAAAAGAAGGC	60	560	474	This study
				MJgidA1R	TGACGCTGTATATCACACGG	60	560	474	This study
enoA	Enolase	G5S_0242	254779–255159	MJenoA1F	CCTATGATGAACCTTATCAATGG	58	431	381	30
				MJenoA1R	TCTTCCTCCGCTAAGCCATCC	58	431	381	30
hemN	Oxygen-independent coproporphyrinogen III oxidase	G5S_0144	150138–150569	MJhemN1F	GATCGCGATAGAGATAGACCC	54	634	432	This study
				MJhemN1R	ATCTTCTCCTGATAGATATCG	54	634	432	This study
fumC	Fumarate hydratase class II	G5S_0015	18169–17705	MJfumC1F	TGATTAAGAAATGTGCAGC	54	572	465	This study
				MJfumC1R	CCTTCAGGTACATTAAGCC	54	572	465	30

^aGenBank accession number CP002608.