Table 1.
rpoB mutations found in first-recurrence sputum samples from Bangladesh and Kinshasa: relative frequency and growth on culture
| Mutation(s) in rpoB sequence | Bangladesh |
Kinshasa |
||
|---|---|---|---|---|
| Frequency [n (%)] | Positive cultures [no. positive/no. testeda (%)] | Frequency [n (%)] | Positive cultures [no. positive/no. testeda (%)] | |
| 508Asn | 1 (0.4) | 0/1 | ||
| 508Ile | 1 (0.6) | 0/1 | ||
| 509Thr&526Leu | 1 (0.6) | 1/1 | ||
| 511Arg&516Gly | 1 (0.4) | 1/1 | ||
| 511Pro | 3 (1.7) | 3/3 | ||
| 511Pro&512Cys | 1 (0.6) | 1/1 | ||
| 511Val | 1 (0.4) | 1/1 | ||
| 513Glu&516Phe | 2 (0.8) | 2/2 | ||
| 513Glu&516Val | 1 (0.4) | 1/1 | ||
| 513Lys | 1 (0.6) | 1/1 | 1 (0.4) | 1/1 |
| 513Lys&526Asp | 2 (1.1) | 2/2 | ||
| 513Pro | 2 (0.8) | 2/2 | ||
| 514Leu&516Val&531Leu | 1 (0.6) | 0/1 | ||
| 515Ile | 1 (0.4) | 0/1 | ||
| 515Ile&533Pro | 1 (0.4) | 1/1 | ||
| 515Thr&516Gly | 1 (0.6) | 1/1 | ||
| 516Asn | 1 (0.6) | 0/1 | ||
| 516Gln | 1 (0.4) | 0/1 | ||
| 516Lys&526Asn | 1 (0.6) | 1/1 | ||
| 516Phe | 2 (1.1) | 2/2 | 2 (0.8) | 1/2 |
| 516Phe&531Leu | 1 (0.6) | 1/1 | ||
| 516Tyr | 4 (2.3) | 4/4 | 5 (2.0) | 4/5 |
| 516Tyr&533Pro | 1 (0.6%) | 1/1 | ||
| 516Val | 6 (3.4) | 3/5 | 18 (7.1) | 11/18 (61) |
| 518Ser | 1 (0.6) | 0/1 | ||
| 522Gln | 8 (3.1) | 8/8 | ||
| 522Leu | 2 (1.1) | 1/2 | 1 (0.4) | 0/1 |
| 523Glu | 1 (0.6) | 0/1 | 1 (0.4) | 0/1 |
| 524Trp&525Pro&DEL526_527 | 1 (0.4) | 0/1 | ||
| 526Arg | 5 (2.9) | 3/4 | 2 (0.8) | 2/2 |
| 526Arg&531Glu | 1 (0.6) | 1/1 | ||
| 526Asn | 2 (1.1) | 2/2 | 4 (1.6) | 2/4 |
| 526Asn&533Pro | 1 (0.4) | 0/1 | ||
| 526Asn&572Leu | 1 (0.6) | 1/1 | ||
| 526Asp | 12 (6.9) | 11/12 (86) | 5 (2.0) | 4/5 |
| 526Leu | 5 (2.9) | 4/4 | 6 (2.4) | 4/6 |
| 526Tyr | 17 (9.7) | 16/17 (94) | 6 (2.4) | 5/6 |
| 531Gln | 1 (0.6) | 0/1 | ||
| 531Leu | 82 (46.9) | 66/77 (86) | 160 (63.0) | 113/157 (72) |
| 531Phe | 1 (0.6) | 0/1 | ||
| 531Trp | 2 (1.1) | 2/2 | 1 (0.4) | 1/1 |
| 533Pro | 7 (4.0) | 5/7 | 7 (2.8) | 5/7 |
| 535Ser | 1 (0.6) | 0/1 | 4 (1.6) | 3/4 |
| 536Ser | 1 (0.6) | 0/1 | ||
| 572Phe | 2 (1.1) | 2/2 | 5 (2.0) | 3/5 |
| DEL509_511 | 3 (1.2) | 1/3 | ||
| DEL513_515 | 3 (1.7) | 3/3 | 2 (0.8) | 0/1 |
| INS511 | 1 (0.6) | 1/1 | ||
| Total no. of isolates with a mutation(s) | 175 (100.0) | 139/167 (83.2) | 254 (100.0) | 177/251 (70.5) |
| No. with disputed resistance | 23 (13.1) | 20/22 (90.9) | 27 (10.6) | 18/27 (66.7) |
| No. with undisputed resistance | 116 (66.3) | 96/110 (87.3) | 173 (68.1) | 124/170 (72.9) |
| No. of isolates with double mutations | 10 (5.7) | 10/10 (100.0) | 6 (2.4) | 5/6 |
| No. of isolates with unusual mutation(s) | 8 (4.6) | 1/8 | 8 (3.1) | 3/8 |
a
Percentages are shown only if the denominator was at least 10.