Fig 1.

Single-step growth curves for EGFP-CSFV and CSFV-Shimen. PK-15 cells were infected with EGFP-CSFV or CSFV-Shimen (multiplicity of infection [MOI], 1) and harvested at 12, 24, 36, 48, 60, and 72 h postinfection. The titers of CSFV-Shimen were determined by IFA using the anti-E2 monoclonal antibody (MAb) 6E10, whereas the titers of EGFP-CSFV were obtained by direct observation of EGFP fluorescence and by IFA. Titers were expressed as log 50% tissue culture infective dose (TCID₅₀)/ml. Data represent the means ± standard deviations (SD) from three independent experiments.