Table 3.
Quantification of Cryptosporidium DNA from 10 natural fecal samples (E1 to E10), five seeded fecal samples (E11 to E15), and suspensions of oocysts in PBS (E16 to E20)
| Sample | No. of oocysts added per g of sample | No. of oocysts detected per g of sample with: |
||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| easyMAG | easyMAG + grinding | Maxwell | MO BIO | NucleoSpin | NucleoSpin + grinding | QIAamp DNA | QIAamp Stool | QIAamp DNA + grinding | ||
| Natural fecal samples | ||||||||||
| E01 | 2.5 | 6 | 4.5 | 0 | 1.5 | 0.5 | 0 | 0 | 1 | |
| E02 | 284.5 | 655 | 293 | 45 | 372 | 278 | 252 | 0.5 | 221 | |
| E03 | 56.5 | 377 | 18.5 | 3 | 565 | 31 | 323 | 2.5 | 280.5 | |
| E04 | 37.5 | 222.5 | 42.5 | 25 | 378.5 | 459 | 178 | 0.5 | 265.5 | |
| E05 | 139 | 1,165 | 192.5 | 0.5 | 422 | 85 | 560 | 0.5 | 550 | |
| E06 | 60.5 | 265.5 | 256.5 | 1.5 | 187 | 1,030 | 327.5 | 3.5 | 151 | |
| E07 | 2,695 | 1,430 | 1,430 | 202 | 3,495 | 1,000 | 5,300 | 198 | 7,200 | |
| E08 | 263 | 175 | 16.5 | 3 | 100 | 6 | 56.5 | 11 | 168.5 | |
| E09 | 2,805 | 7,450 | 2,140 | 575 | 5,100 | 496 | 27,700 | 174 | 20,000 | |
| E10 | 1,745 | 5,950 | 1,935 | 318 | 20,600 | 3,150 | 59,000 | 427 | 27,550 | |
| Seeded fecal samples | ||||||||||
| E11 | 0.5 | 0 | 2 | 5 | 0.5 | 0.5 | 0.5 | 0.5 | 0 | 2.5 |
| E12 | 1 | 0 | 0.5 | 0 | 0 | 5.5 | 0 | 2 | 0 | 4 |
| E13 | 2.5 | 0.5 | 4.5 | 2 | 0 | 11 | 0.5 | 15 | 0 | 13 |
| E14 | 5 | 2.5 | 8.5 | 18.5 | 0.5 | 8.5 | 2 | 12.5 | 0 | 16.5 |
| E15 | 50 | 135 | 148.5 | 76 | 3 | 116.5 | 2.5 | 36 | 3 | 26 |
| Seeded PBS samples | ||||||||||
| E16 | 0.5 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 2.5 |
| E17 | 1 | 1 | 0 | 1 | 0 | 0 | 0 | 3.5 | 0 | 1 |
| E18 | 2.5 | 0 | 2.5 | 0.5 | 0 | 1.5 | 0.5 | 7.5 | 0 | 9.5 |
| E19 | 5 | 4 | 0.5 | 0 | 0 | 1.5 | 0.5 | 29 | 0 | 19 |
| E20 | 50 | 43 | 20.5 | 38.5 | 0.5 | 10 | 1.5 | 134 | 0 | 147.5 |