Table 5.
Influence of multiplexing on the main characteristics of the qPCR
| DNA species | Probe(s)a | Slope | y intercept |
|---|---|---|---|
| C. hominis | Pan-crypto | −3.32 | 40.27 |
| C. hominis | Pan-crypto + C. parvum | −3.44 | 40.60 |
| C. hominis | C. hominis + C. parvum | −3.54 | 40.01 |
| C. hominis | C. hominis | −3.46 | 39.65 |
| C. parvum | Pan-crypto | −3.41 | 39.63 |
| C. parvum | Pan-crypto + C. parvum | −3.53 | 39.46 |
| C. parvum | C. hominis + C. parvum | −3.4 | 39.72 |
| C. parvum | C. parvum | −3.42 | 39.45 |
a
The combination of the Pan-crypto and C. hominis probes was not tested because the probes used the same fluorescent ligand.