Table 1.
Results of T. gondii serological and molecular diagnosis and follow-up
| Sampling day vs HSCT | Serologya |
T. gondii burden (parasites/ml)b |
|||||||
|---|---|---|---|---|---|---|---|---|---|
| IgG |
IgM |
||||||||
| ELISA (IU/ml) | HSDA (titer) | TXG (IU/ml) | TXGA index | ELISA absorbance | ISAgA index | TXM index | Blood | BAL fluid | |
| −21 | 578 | ND | ND | ND | neg. | ND | neg. | ND | ND |
| +21 | ND | 1:640 | 144 | 0.566 | ND | 3 | 0.09 | 15,000 | 100,000 |
| +30 | ND | 1:1,280 | 287 | ND | ND | 0 | 0.10 | 200 | ND |
a
Positivity thresholds for IgG, ≥5 IU/ml for ELISA (Enzygnost Toxo IgG, Siemens), ≥1:40 for HSDA, and ≥8 IU/ml for TXG Vidas (bioMérieux). For IgM, ELISA (Enzygnost Toxo IgM, Siemens) absorbance values, ≥0.3; TXM Vidas (bioMérieux) index, ≥0.65; ISAgA (bioMérieux) index, 9 to 12. For TXGA Vidas (bioMérieux), low index (I), I < 0.200; intermediate index, 0.200 ≤ I < 0.300; high index, I ≥ 0.300. ND, not done; neg., negative.
b
Number of parasites/ml of sample quantified by real-time PCR.