Amino acid sequence alignment of T. vaginalis α-actinin with the amino acid sequences of proteins from representative organisms and HuACTN1 (see Materials and Methods). The epitopes identified with pooled positive control sera from women and men, as indicated in Table 1, are boxed. Red foreground, gaps in the sequence; red lettering, 0 to 33% identity; black lettering, approximately 34 to 67% identity; blue lettering, 68 to 100% identity.