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. 2013 Aug;51(8):2483–2490. doi: 10.1128/JCM.00582-13

Table 1.

T. vaginalis α-actinin epitope mapping using MAb HA423 and positive control sera from women and men

Spot no. Amino acids Epitope sequence Epitope designation for reaction with:
Positive control sera from womena Positive control sera from mena MAbb
2 4–14 RREGLLDDAWE W1
11–12 34–41 IQFETIET W2
21–23 67–71 KQPKM W3
50 148–158 YEHVAVNNFTT W4
69 205–215 YVYLDPEDVID W5
80–82 244–248 ADKIK W6
97–99 295–302 RGKLASVI W7
111–112 334–341 NRPIPEIP W8
152–153 457–464 HHSQLITY W9 M1
165–166 496–503 YDEAIAFK W10 M2
214–216 646–650 KLNYK W11 M3
215–217 649–653 YKVTY W12 M4 HA423
268–270 808–812 KYFDK W13 M5
a

Positive control sera from female patients with trichomonosis and positive control sera from men were highly reactive by ELISA with purified recombinant ACT-P2 immobilized on microtiter plates, by whole-cell ELISA with organisms on microtiter wells, as described previously (27, 29, 39), and by immunoblotting (Fig. 1).

b

MAb HA423 was generated to trichomonad α-actinin using T. vaginalis organisms with standard protocols, as done for the generation of other MAbs (5, 9, 12, 29).