Table 1.

T. vaginalis α-actinin epitope mapping using MAb HA423 and positive control sera from women and men

Spot no.	Amino acids	Epitope sequence	Epitope designation for reaction with:
			Positive control sera from womena	Positive control sera from mena	MAbb
2	4–14	RREGLLDDAWE	W1
11–12	34–41	IQFETIET	W2
21–23	67–71	KQPKM	W3
50	148–158	YEHVAVNNFTT	W4
69	205–215	YVYLDPEDVID	W5
80–82	244–248	ADKIK	W6
97–99	295–302	RGKLASVI	W7
111–112	334–341	NRPIPEIP	W8
152–153	457–464	HHSQLITY	W9	M1
165–166	496–503	YDEAIAFK	W10	M2
214–216	646–650	KLNYK	W11	M3
215–217	649–653	YKVTY	W12	M4	HA423
268–270	808–812	KYFDK	W13	M5

^aPositive control sera from female patients with trichomonosis and positive control sera from men were highly reactive by ELISA with purified recombinant ACT-P2 immobilized on microtiter plates, by whole-cell ELISA with organisms on microtiter wells, as described previously (27, 29, 39), and by immunoblotting (Fig. 1).

^bMAb HA423 was generated to trichomonad α-actinin using T. vaginalis organisms with standard protocols, as done for the generation of other MAbs (5, 9, 12, 29).