Fig 1.

Schematic view of magnetic nanobead sensitized with soluble PV receptor. Original magnetic nanobead (FG bead; left), magnetic nanobead coated with protein A/G (middle), and PVR-IgG2a-sensitized magnetic nanobead (right) are shown with protein A/G, PVR-IgG2a, and PV of actual size. Protein A/G is a recombinant chimeric protein that consists of 4 Fc-binding domains of protein A and 2 Fc-binding domains of protein G. Bar, 200 nm. Approximately 1,600 molecules of protein A/G were immobilized on a magnetic nanobead. The concentration of the magnetic beads used in this study was 1.8 × 10¹² beads per ml.