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. 2013 Aug;51(8):2717–2720. doi: 10.1128/JCM.00499-13

Fig 3.

Fig 3

Extraction of PVpv and endogenous PV from the stool extracts by PVR-IgG2a-sensitized magnetic nanobeads. (A) Extraction of PV1pv exogenously added to the stool extracts derived from polio cases. The indicated amounts of the beads were added to the stool extracts containing PV1pv (10,000 IU in 100 μl) and were then incubated at 4°C for 1 h. The beads were collected with a magnetic separator, and then the titer of PV1pv remaining in the supernatant was measured. Infectivity of PV1pv in the stool extracts without the beads is also shown (black bars). The efficiency of extraction (%) by the beads and PV1pv infection (%) in the stool extracts (the final concentration of the stool extracts was 5% [vol/vol]) are shown. The infectivity of PV1pv in the cell culture medium without the stool extracts was taken as 100%. (B) Extraction of endogenous PVs from the stool extracts derived from polio cases. PV-positive stool extracts were treated with or without the indicated amounts of the beads, and then the residual infectivity of the extracts was analyzed in L20B cells. The cells were observed for CPE for 5 days after inoculation. CPE scores of +1, +2, +3, and +4 indicate the appearance of CPE in approximately 25, 50, 75, and 100% of the cells, respectively. PV1(Sabin) of the indicated titer was inoculated into the cells as a control. p.i., postinfection. (C) Extraction of PV3pv exogenously added to the stool extracts derived from AFP cases. Four microliters of the beads was added to the stool extracts containing PV3pv (20,000 IU in 100 μl) and was then incubated at 4°C for 1 h. The beads were collected with a magnetic separator, and then the titer of PV3pv remaining in the supernatant was measured. Infectivity of PV3pv in the stool extracts without the beads is also shown. The efficiency of extraction (%) by the beads and PV3pv infection (%) in the stool extracts without the beads (the final concentration of the stool extracts was 5% [vol/vol]) are shown. The infectivity of PV3pv in the cell culture medium without the stool extracts was taken as 100%. The numbers of the samples above or below 90% extraction are shown.