Table 1.
Isolate | Disc diffusion (mm/susceptibility) | Etest (MIC [μg/ml]/susceptibility) | Agar dilution (MIC [μg/ml]/susceptibility) | Vitek 0.6 McF (MIC [μg/ml]/susceptibility)b | Broth microdilution (MIC [μg/ml]/susceptibility) | aac(3)-IIa genec | DiversiLab clone |
---|---|---|---|---|---|---|---|
7 | 0/R | 256/R | 128/R | ≥16/R | >256/R | − | D |
40 | 4/R | 8/I | 4/S | 4/S | >256/R | + | C |
3 | 3/R | 32/R | 16/R | 8/I | 256/R | − | D |
4 | 10/R | 16/R | 8/I | 8/I | 256/R | − | C |
27 | 0/R | 64/R | 32/R | ≥16/R | 256/R | − | D |
31 | 12/R | 16/R | 16/R | 4/S | 256/R | + | C |
18 | 13/I | 16/R | 4/S | 8/I | 128/R | + | C |
36 | 13/I | 8/I | 16/R | 4/S | 128/R | + | C |
38 | 0/R | 64/R | 16/R | ≥16/R | 128/R | − | A |
10 | 13/I | 16/R | 4/S | 8/I | 64/R | + | C |
5 | 11/R | 8/I | 8/I | 8/I | 32/R | + | C |
6 | 14/I | 4/S | 2/S | 2/S | 32/R | + | C |
11 | 13/I | 16/R | 4/S | 2/S | 32/R | − | C |
12 | 9/R | 32/R | 8/I | 8/I | 32/R | + | C |
13 | 11/R | 32/R | 4/S | 4/S | 32/R | + | C |
17 | 13/I | 16/R | 2/S | 4/S | 16/R | + | C |
22 | 16/S | 8/I | 2/S | ≤1/S | 16/R | + | C |
23 | 21/S | 2/S | ≤0.5/S | ≤1/S | 16/R | − | B |
39 | 15/S | 8/I | 2/S | 2/S | 16/R | + | C |
8 | 13/I | 8/I | 4/S | 4/S | 8/I | + | C |
9 | 15/S | 8/I | 2/S | 2/S | 8/I | + | C |
14 | 14/I | 8/I | 4/S | 2/S | 8/I | + | C |
15 | 15/S | 8/I | 4/S | 4/S | 8/I | + | C |
20 | 15/S | 4/S | 2/S | ≤1/S | 8/I | + | C |
26 | 0/R | 64/R | 2/S | ≥16/R | 8/I | + | C |
32 | 17/S | 4/S | 2/S | ≤1/S | 8/I | + | C |
33 | 10/R | 16/R | 8/I | 4/S | 8/I | + | C |
42 | 14/I | 8/I | 4/S | 2/S | 8/I | + | C |
29 | 14/I | 8/I | 2/S | 2/S | 4/S | − | C |
24 | 22/S | 2/S | ≤0.5/S | ≤1/S | 2/S | − | C |
35 | 21/S | 2/S | ≤0.5/S | ≤1/S | 2/S | − | B |
2 | 19/S | 2/S | ≤0.5/S | ≤1/S | 1/S | − | B |
16 | 23/S | 2/S | ≤0.5/S | ≤1/S | 1/S | − | A |
21 | 15/S | 2/S | ≤0.5/S | ≤1/S | 1/S | − | A |
28 | 23/S | 1/S | ≤0.5/S | ≤1/S | 1/S | + | A |
30 | 20/S | 2/S | ≤0.5/S | ≤1/S | 1/S | − | A |
34 | 23/S | 1/S | ≤0.5/S | ≤1/S | 1/S | − | A |
37 | 21/S | 2/S | ≤0.5/S | ≤1/S | 1/S | − | B |
41 | 21/S | 2/S | ≤0.5/S | ≤1/S | 1/S | − | C |
PCR of aac(C)-IIa and DiversiLab results. S, sensitive; I, intermediate, R resistant.
Shading indicates very major errors; underlining indicates minor errors.
+, aac(3)-IIa gene present; −, aac(3)-IIa gene absent.