Fig 4.

Innate immune-related genes overexpressed in cancer are suppressed in telomere-elongated xenograft tumors. (A) Altered gene expression profiles due to telomere elongation in PC-3 xenografts, with (upper white segments) or without (lower black segments) hTERT overexpression. With changes of >2.0-fold, expression of 190 probes (151 genes) was changed irrespective of hTERT overexpression/excision. For each probe, at least one xenograft had a signal value of >100. Detailed information is provided in Data set S1 in the supplemental material. (B) The RNA expression of three genes was quantified using real-time PCR. Error bars indicate the standard deviations (n = 3). The expression value for each gene was normalized to that of β-actin. There were significant differences in each combination of control (mock or mock/cre+) and telomere-elongated (LhTERTL or LhTERTL/cre+) xenograft tumors (P < 0.05 by the two-tailed t test). (C) Immunohistochemical staining of STAT1, ISG15, and OAS3. Original magnification, ×200. (D) Summary of disease analyzed using the Oncomine database, which indicates the number of significant unique microarray results with changes of >2.0-fold (P < 0.0001 by the two-tailed t test) with increased (red) or decreased (blue) expression in tumor relative to normal tissue.