Fig 6.

miR-33 modulation of genes involved in glucose homeostasis. (A) qRT-PCR analysis of genes involved in glycolysis, including GCK, ALDO, BPGM, FBP/PFK, and PKLR, in HepG2 cells transfected with miR-33b. ABCA1 was included as a positive control. (B) qRT-PCR analysis of genes involved in glycogen metabolism, including GYG, PGM, and PYGL, in HepG2 cells transfected with miR-33b. Data are expressed as relative expression levels and correspond to the means ± SEM from 3 independent experiments performed in triplicate. ∗, significantly different from cells transfected with CM (normalized to 1) (dashed line). (C) qRT-PCR analysis of PGM expression in HepG2 cells transfected with control miRNA (CM) or miR-33b mimic (miR-33b) and treated with DXM and Bt₂-cAMP during 2, 4, and 6 h in glucose-free medium in the absence of lactate or pyruvate. Data are presented as means ± SEM from 3 independent experiments performed in triplicate. ∗, P ≤ 0.05 versus untreated cells at each time point. (D) Glycogen content in HepG2 cells transfected with CM versus miR-33b. Data are presented as means ± SEM from 3 independent experiments performed in triplicate. ∗, significantly different from cells transfected with CM (P ≤ 0.05).