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Phosphomimetic alteration of Ser at position 77 of L13a to Asp abrogates ribosome incorporation of L13a. HEK 293T cells were transfected with Flag-tagged wild-type L13a (a) and/or its mutant variant S77D (b). Total protein from different ribosomal fractions, resolved by sucrose density gradient centrifugation (10 to 50% sucrose), was analyzed by Western blotting with anti-Flag antibody.
