Fig 4.

Representative effects of various drugs against A. castellanii cysts. A. castellanii cysts were scraped from nonnutrient agar plates and incubated with various drugs (500 μg per ml) for 24 h. After this incubation, the cysts were washed three times with PBS and reinoculated in fresh PYG medium at 30°C for up to 48 h. When treated with 500 μg per ml of amlodipine, prochlorperazine, and loperamide, A. castellanii cysts did not reemerge as viable A. castellanii trophozoites. Viable A. castellanii trophozoites emerged in control wells, as well as with all other drugs. The results are representative of three independent experiments.