Table 4.
norA-related genotypes in clinical isolates of S. aureus
| Intergenic region or parent and mutant strain name | Presence of: |
MICc (mg/liter) |
Sequence of polymorphic site in norA promoter regiona | Commentb | |||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| qacA | qacB | qacC | qacG | NOR | CIP | EB | BZC | CHX | |||
| Intergenic region | |||||||||||
| 1 | 1111111111---------------99999988643322228761 | ||||||||||
| 2 | 95433221009754107415639820 | ||||||||||
| 3 | 4052062270 | ||||||||||
| Strain | |||||||||||
| MW2 | − | − | − | − | ND | 0.5 | 8 | 1 | 2 | TAT-ATAGAT---------------AAAATTTGCGCTCATGGTGT | wt allele |
| Mu50 | + | − | − | − | ND | 64 | 256 | 4 | 4 | ...-......---------------.................... | wt allele |
| COL | + | − | − | − | ND | 64 | 128 | 4 | 4 | ...-......---------------.................... | wt allele |
| RN4220 | − | − | − | − | 1 | 0.5 | 8 | 2 | 8 | ...-......---------------.................... | wt allele |
| ATCC 6538 | − | − | − | − | 1 | 0.5 | 4 | 2 | 4 | ...-......---------------.................... | wt allele |
| 1016 | − | − | − | − | ND | 0.25 | 8 | 2 | 2 | ...-......---------------.................... | wt allele |
| 1025 | − | − | − | − | ND | 1 | 8 | 2 | 2 | ...-......---------------.................... | wt allele |
| 1130 | − | − | − | − | ND | 0.25 | 32 | 2 | 2 | ...-......---------------.................... | wt allele |
| 1170 | − | − | − | − | 64 | 64 | 4 | 8 | 2 | ...-......---------------.................... | wt allele |
| 1173 | + | − | − | − | 64 | 64 | 256 | 4 | 2 | ...-......---------------.................... | wt allele |
| 1177 | − | − | − | − | 64 | 64 | 8 | 1 | 2 | ...-......---------------.................... | wt allele |
| 1192 | + | − | − | − | ND | 64 | 256 | 8 | 4 | ...-......---------------.................... | wt allele |
| 1205 | + | − | − | − | 64 | 0.5 | 256 | 8 | 2 | ...-......---------------.................... | wt allele |
| 1209 | − | − | + | − | 8 | 0.25 | 32 | 8 | 2 | ...-......---------------.................... | wt allele |
| 1219 | + | − | − | − | ND | 8 | 256 | 8 | 4 | ...-......---------------.................... | wt allele |
| 1236 | − | − | − | − | 2 | 0.25 | 16 | 8 | 2 | ...-......---------------.................... | wt allele |
| 1284 | − | − | − | − | ND | 0.25 | 8 | 2 | 1 | ...-......---------------.................... | wt allele |
| 1299 | − | − | + | − | 2 | 0.25 | 16 | 4 | 1 | ...-......---------------.................... | wt allele |
| 1390 | − | − | + | − | 64 | 2 | 32 | 8 | 1 | ...-......---------------.................... | wt allele |
| 1407 | − | − | − | − | ND | 0.25 | 2 | 1 | 1 | ...-......---------------.................... | wt allele |
| 1503 | + | − | − | − | ND | 8 | 128 | 4 | 2 | ...-......---------------.................... | wt allele |
| 1505 | + | − | − | − | ND | 8 | 128 | 4 | 4 | ...-......---------------.................... | wt allele |
| 1508 | + | − | − | − | ND | 8 | 128 | 4 | 2 | ...-......---------------.................... | wt allele |
| 1522 | + | − | − | − | ND | 8 | 128 | 4 | 2 | ...-......---------------.................... | wt allele |
| 1619 | − | − | − | − | 2 | 0.25 | 4 | 2 | 2 | ...-......---------------.................... | wt allele |
| 1730 | − | − | − | − | 32 | 8 | 4 | 8 | 4 | ...-......---------------.................... | wt allele |
| 1828 | − | + | − | − | 2 | 0.25 | 256 | 8 | 2 | ...-......---------------.................... | wt allele |
| 1889 | − | − | + | − | 64 | 64 | 64 | 8 | 1 | ...-......---------------.................... | wt allele |
| 2092 | − | − | + | − | ND | 0.25 | 32 | 8 | 2 | ...-......---------------.................... | wt allele |
| 2106 | + | − | − | − | 64 | 8 | 128 | 8 | 4 | ...-......---------------.................... | wt allele |
| 2363 | − | − | − | − | ND | 64 | 32 | 4 | 2 | ...-......---------------.................... | wt allele |
| 2391 | − | − | − | − | 64 | 32 | 4 | 8 | ...-......---------------.................... | wt allele | |
| 2507 | − | + | − | − | 1 | 0.25 | 512 | 8 | 2 | ...-......---------------.................... | wt allele |
| 2577 | − | − | − | − | 2 | 0.25 | 4 | 64 | 8 | ...-......---------------.................... | wt allele |
| 2628 | + | − | − | − | 64 | 0.25 | 128 | 8 | 8 | ...-......---------------.................... | wt allele |
| 2671 | + | − | − | − | 2 | 0.25 | 128 | 8 | 4 | ...-......---------------.................... | wt allele |
| 1191 | − | − | − | + | 128 | 0.25 | 64 | 4 | 2 | ...-....G.---------------.................... | A107G, known mutation (9) |
| ATCC 25923 | − | − | − | − | 1 | 1 | 16 | 2 | 2 | ...A......---------------........T.A.A....... | wt allele |
| 1285 | − | − | − | − | 64 | 0.25 | 8 | 2 | 2 | ...A......---------------........T.A.A....... | wt allele |
| 1027 | − | − | − | − | 128 | 0.25 | 16 | 16 | 4 | G..-......---------------.................... | T194G, new mutation |
| 1158 | + | − | − | − | 128 | 8 | 64 | 8 | 4 | G..-......---------------.................... | T194G, new mutation |
| 1614 | − | − | + | − | 64 | 2 | 64 | 8 | 2 | .GC-......---------------.................... | A150G, T145G, new mutations |
| 1387 | − | − | − | − | 64 | 16 | 4 | 4 | 2 | ...-......---------ATCAAT................---. | Known duplication (11), new deletion |
| 1607 | − | − | − | − | 64 | 32 | 16 | 4 | 2 | ...-......GTTGTAATACAATAT.................... | New duplication |
| 1881 | − | − | − | − | 64 | 64 | 32 | 8 | 4 | ...-......-----------CAAT.................... | Known duplication (11) |
| 1891 | − | − | − | − | 64 | 64 | 32 | 8 | 4 | ...-......-----------CAAT.................... | Known duplication (11) |
| 1939 | − | − | − | − | 64 | 64 | 32 | 8 | 4 | ...-......-----------CAAT.................... | Known duplication (11) |
| 1951 | − | − | − | − | 64 | 64 | 32 | 4 | 4 | ...-......-----------CAAT.................... | Known duplication (11) |
| 1878 | − | − | − | − | 64 | 64 | 8 | 4 | 2 | ...-...A..-----------CAAT.........A.GAGAT...A | Known duplication (11) |
| 1894 | + | − | − | − | 64 | 64 | 16 | 4 | 2 | ...-...A..-----------CAAT.........A.GAGAT...A | Known duplication (11) |
| 2345 | − | − | + | − | 16 | 0.25 | 32 | 2 | 2 | ...-...A..---------------.........A.GAGAT...A | wt allele |
| 2635 | − | − | − | − | 8 | 0.5 | 128 | 8 | 4 | ...A..TA..-------------—T.........A..AGAT...A | New duplication; A122T, new mutation |
| 2605 | − | − | − | − | 16 | 64 | 64 | 4 | 2 | ...A..TA..---------------.........A..AGAT...A | A122T, new mutation |
| 2634 | − | − | − | − | 8 | 0.5 | 32 | 8 | 4 | ...A..TA..---------------.TCTAAG-.A..AGAT...A | T91A, known mutation (22); A122T, A94T, T89A, new mutations; new deletion |
| 1277 | − | − | − | − | 64 | 0.5 | 8 | 2 | 2 | ...A...A..---------------.........A..AGAT...A | wt allele |
a
Polymorphic sites are indicated with respect to the norA promoter region sequence of S. aureus MW2. The first three rows provide the numbering of the intergenic regions. The intergenic regions start from the nucleotide upstream of the norA start codon (NC_003923 position 739144) and are numbered backward (from right to left). Nucleotides that were the same as those of MW2 in all sequences are not shown. Dots indicate perfect homology with the reference sequence. Insertions and deletions are marked with dashes. Mutations probably involved in norA overexpression are in boldface.
b
wt, wild type.
c
ND, not determined.