Fig 1.

Characterization of anti-HIV-1 activity and cytotoxicity of AH0109. (A) Chemical structure of AH0109. (B) Determination of the 50% effective concentration (EC₅₀) of AH0109 against HIV-1 pNL4.3-GFP⁺ virus infection (MOI of 0.1) in 0.5 × 10⁶ C8166 T cells. (C) At 3 days postinfection with pNL4.3-GFP⁺ virus, the infected C8166 T cells (GFP-positive) in the presence or absence of AH0109 were visualized by fluorescence microscopy. (D) Determination of 50% cell culture inhibitory dose (CCID₅₀) of AH0109 in C8166 T cells by using WST-1 cell proliferation and a cytotoxicity assay kit (Roche). The results in panels B and D are representative of three independent experiments, respectively.