Fig 4.

Energetically minimized structures of TEL binding to the 50S subunit of the ribosome containing unmethylated (A) and methylated (B) G748. Residues 748 to 752 in hairpin 35 are represented by a ribbon model (blue). Ribosomal protein L22 is shown by a tube model (green). TEL is represented by a ball-and-stick model. Dashed lines show the distances from the center of the aromatic ring of the alkyl-aryl chain of TEL to the center of each aromatic ring of A752 (a and b) and to the carbon atom of the methyl group at the N-1 position of G748 (c) and from a hydrogen atom of the amino group at the N-6 position of A2058 to the nitrogen atom (d) and to the oxygen atom of the hydroxyl oxygen group (e) on the cladinose sugar of TEL.