Table 2.
Plasmids used in this study
| Plasmid | Relevant characteristics | Reference or source |
|---|---|---|
| pUC18 | Cloning vector | Laboratory collection |
| pT7Blue | Cloning vector | Novagen |
| pLZ12-Km2 | Shuttle vector | 55 |
| pTKY858 | pT7Blue with 618-bp erm(B) fragment | 31 |
| pTKY862 | pLZ12-Km2 with aad(9) Sp resistance cassette | 31 |
| pTKY1041 | pLZ12-Km2 with 1,401-bp erm(B) fragment from S1 | This study |
| pTKY1042 | pT7Blue with disrupted erm(B) fragment by insertion of aad(9) Sp resistance cassette | This study |
| pTKY1109 | pUC18 with 328-bp tlrB fragment | This study |
| pTKY1110 | pUC18 with disrupted rlmAII fragment by insertion of an aad(9) Sp resistance cassette | This study |
| pTKY1111 | pLZ12-Km2 with 1,065-bp tlrB fragment from S1 | This study |