Table 3.
Mutations in RlmAII proteins of TEL-resistant S. pneumoniae strains
| Mutation in RlmAII | Strain | MICa (μg/ml) |
Level (%) of dimethylation at A2058b |
||
|---|---|---|---|---|---|
| −ERY | +ERY | −ERY | +ERY | ||
| Wild type | S1 | 2 | 2 | 71.0 ± 8.4 | 76.7 ± 9.7 |
| Cys23Arg (67tGT69 → cGT) | Sp44 | 32 | 32 | 87.5 ± 5.6 | 81.9 ± 13.2 |
| Cys36Arg (106tGC108 → cGC) | Sp47 | 32 | 32 | 77.3 ± 2.2 | 66.7 ± 29.7 |
| Leu113Pro (337CtA339 → CcA) | Sp42, Sp43 | 32 | 32 | 90.4 ± 7.7 | 82.6 ± 16.4 |
| Ala135Thr (403gCT405 → aCT) | Sp39 | 32 | 32 | 89.8 ± 7.9 | 75.7 ± 17.2 |
| Leu164Pro (490CtG491 → CcG) | Sp33 | 16 | 16 | 69.2 ± 6.3 | 94.2 ± 9.4 |
| Leu165Pro (492CtT494 → CcT) | Sp37 | 32 | 32 | 79.8 ± 17.5 | 70.8 ± 7.2 |
| Arg200Cys (598cGT600 → tGT) | Sp36, Sp46 | 32 | 32 | 82.5 ± 9.0 | 80.8 ± 14.8 |
| Tyr212Cys (634TaT636 → TgT) | Sp32, Sp34 | 16 | 32 | 91.2 ± 5.7 | 81.7 ± 11.4 |
| Leu248Pro (739CtA741 → CcA) | Sp45 | 32 | 32 | 77.1 ± 7.8 | 80.27 ± 7.5 |
| Trp142stop codon (424TgG426 → TaG) | Sp38 | 16 | 16 | 75.8 ± 11.4 | 80.9 ± 10.0 |
| Frame shift by insertion of an A between nt 355 and 356 | Sp35, Sp40, Sp41 | 32 | 32 | 87.9 ± 7.9 | 82.6 ± 8.8 |
| ΔRlmAII | Sp274 | 16 | 16 | 73.7 ± 10.0 | 85.7 ± 11.5 |
a
Values for at least 3 independent experiments are given.
b
Quantification of the band corresponding to dimethylation at A2058 relative to the total band. Mean values and standard deviations for at least 3 independent experiments are given.