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Effect of lytSR and lrgAB on expression of the mprF and dlt genes. RNA samples were isolated from mid-exponential-growth-phase cultures of the strain set, and were subjected to qRT-PCR to detect and quantify transcription of mprF (A), dltA (B), and lrgA (C). The mid-exponential time point has been determined based on our pilot studies showing that expression of mprF and dlt is maximal at this time point. Comp, complemented strain.
